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microbeads mouse anti cd14 (Miltenyi Biotec)

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Miltenyi Biotec microbeads mouse anti cd14
Microbeads Mouse Anti Cd14, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 4689 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+human+cd14+antibodies/CD14+MicroBeads%2C+human/pmc12992950-2-0-10
Average 98 stars, based on 4689 article reviews

microbeads mouse anti cd14 - by Bioz Stars, 2026-10

98/100 stars

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Isolation:

Article Title: NcROP2 deletion reduces Neospora caninum virulence by altering parasite stage differentiation and hijacking host immune response
Article Snippet: Briefly, peripheral blood mononuclear cells (PBMCs) were isolated through density gradient centrifugation with Histopaque 1077 (Sigma-Aldrich, St. Louis, MO, USA). .. Subsequently, monocytes were isolated using mouse anti-human CD14 antibodies linked to microbeads (Miltenyi Biotec Ltd., San Diego, CA, USA), following the manufacturer’s instructions. .. Monocytes were seeded in 6-well plates at a density of 3×10 6 cells/well and cultured in RPMI 1640 medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% heat-inactivated fetal calf serum (FCS), 50 μg/ml gentamicin, 2mM L-glutamine, 50 μM β-mercaptoethanol and 20 mM HEPES.

Purification:

Article Title: Loss of NcBPK1 impairs bradyzoite differentiation and enhances virulence in Neospora caninum
Article Snippet: In brief, peripheral blood mononuclear cells (PBMCs) were separated using density gradient centrifugation with Histopaque 1077 (Sigma-Aldrich, St. Louis, MO, USA). .. Monocytes were then purified using mouse anti-human CD14 antibodies conjugated to microbeads (Miltenyi Biotec Ltd., San Diego, CA, USA), according to the manufacturer’s guidelines. .. Isolated monocytes were plated in 6-well plates at a density of 3 × 10 6 cells per well and cultured in RPMI 1640 medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% heat-inactivated fetal calf serum (FCS), 50 μg/ml of gentamicin, 2 mM of L-glutamine, 50 μM of β-mercaptoethanol, and 20 mM of HEPES (all from Thermo Fisher Scientific).

Article Title: Loss of NcBPK1 impairs bradyzoite differentiation and enhances virulence in Neospora caninum.
Article Snippet: In brief, peripheral blood mononuclear cells (PBMCs) were separated using density gradient centrifugation with Histopaque 1077 (Sigma-Aldrich, St. Louis, MO, USA). .. Monocytes were then purified using mouse anti-human CD14 antibodies conjugated to microbeads (Miltenyi Biotec Ltd., San Diego, CA, USA), according to the manufacturer’s guidelines. .. Isolated monocytes were plated in 6-well plates at a density of 3 × 106 cells per well and cultured in RPMI 1640 medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% heat-inactivated fetal calf serum (FCS), 50 μg/ml of gentamicin, 2 mM of L-glutamine, 50 μM of β-mercaptoethanol, and 20 mM of HEPES (all from Thermo Fisher Scientific).

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microbeads mouse anti cd14 (Miltenyi Biotec)

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